A FLUOROMETRIC METHOD FOR THE ESTIMATION OF TRYPTOPHAN
نویسندگان
چکیده
منابع مشابه
A fluorometric method for the estimation of tryptophan.
The various calorimetric methods now employed for the estimation of tryptophan are not specific for tryptophan but give colored products with many compounds containing the indole nucleus, including indole itself. In order to facilitate studies of the enzymatic synthesis of tryptophan from indole and serine by extracts of Neurospora (l), a search for a rapid, quantitative method for the estimati...
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Interference of sucrose with the fluorometric assay of tryptophan.
Sucrose has been found to decrease the fluorescence observed during the standard fluorometric assay of tryptophan. The quenching is enhanced with increasing concentrations of sucrose. This phenomenon is not due to a metal contaminant in the sucrose, nor to a direct interaction of the sugar with the fluorophore, norharman, which results from the oxidation of a tryptophanformaldehyde complex by F...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1949
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51220-0